DNA isolation / purification Cells Immortalized cell lines

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-468

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-231

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-157

Get tips on using ReliaPrep™ RNA Miniprep Systems to perform RNA isolation / purification Cells - immortalized MDA-MB-157

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using Aurum™ Total RNA Mini Kit to perform RNA isolation / purification Cells - immortalized bEnd.3

Get tips on using RNA Stat-60 RNA Extraction Reagent to perform RNA isolation / purification Cells - immortalized HEK 293

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized A2780

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized HeLa