rna-isolation-purification-cells-primary-mouse-cortical-neurons

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3

Get tips on using Mini Total RNA Kit to perform RNA isolation / purification Cells - immortalized KG-1

Get tips on using Absolutely RNA miRNA Kit to perform RNA isolation / purification Cells - immortalized HT-29

Get tips on using ZymoBIOMICS RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized HT-1080

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Muscle

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - Mouse Lung