siRNA / miRNA gene silencing Human Huh7

Get tips on using ON-TARGETplus Mouse Nr1h2 (22260) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - M210B4 LXR‐β/Nr1h2

Get tips on using ON-TARGETplus Mouse Zbp1 (58203) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Zbp1/Dai

Get tips on using Silencer® Select_FPr1/Silencer® Select_FPr2 siRNA to perform siRNA / miRNA gene silencing Mouse - Neuro 2a Fpr1/Fpr2

Cell cytotoxicity assays measure the ability of certain compounds or chemical mediators to reduce the viability of the cells. The term cell cytotoxicity assay can sometimes be used interchangeably with cell proliferation assay. Healthy living cells can be identified by the use of formazan dyes, protease biomarkers or by measuring ATP content. The formazan dyes are chromogenic products formed by the reduction of tetrazolium salts by dehydrogenases, such as lactate dehydrogenase (LDH) and reductases that are released during cell death. Common tetrazolium salts include INT, MTT, MTS and XTT. Cell cytotoxicity can also be measured by using the SRB and WST-1 assays. These assays can usually be used in a high-throughput fashion and can be quantitated by measuring absorbance, colorimetry or luminescence. All these assays require similar numbers of cell plating at the initiation, a time course of treatment with the cytotoxic agent and at least triplicates for each condition at every point of analysis. Cell shrinkage, plasma membrane blebbing, cell detachment, externalization of phosphatidylserine, nuclear condensation and ultimately DNA fragmentation are well-described features of apoptosis. The assays that rely on cell membrane integrity for their function, may not be able to quantify early apoptosis. Therefore, in order to distinguish early apoptotic vs. late apoptotic or necrotic cells, additional flow cytometry techniques can be used. A combination of Annexin V and PI (propidium iodide) can be used to distinguish early (Annexin V+/PI-) and late apoptotic (Annexin V+/PI+) cells. Sometimes, caspase assays are used in order to differentiate the stages of apoptosis.

Get tips on using Orai1 Rat siRNA Oligo Duplex (Locus ID 304496) to perform siRNA / miRNA gene silencing Rat - RBL-2H3 Orai1

Get tips on using ON-TARGET plus Mouse Becn1 (56208) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 4T1 BECN-1

Get tips on using ON-TARGETplus Rat Rac1 (363875) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Rat - MTLn3 Rac1

Get tips on using SMARTpool: ON-TARGETplus Hipk2 siRNA to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

Get tips on using SignalSilence® NF-κB p65 siRNA I #6261 to perform siRNA / miRNA gene silencing Rat - H9c2 NF-κB RelA (p65)

Get tips on using pcDNA™3.1 (+) Mammalian Expression Vector to perform siRNA / miRNA gene silencing Human - U251 cofilin-1 (CFL1)