Immunohistochemistry Collagen VII antibody [LH7.2]

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using MHC Class II (I-A/I-E) Monoclonal Antibody (M5/114.15.2), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - MHCII

Get tips on using PDGF Receptor β (28E1) Rabbit mAb to perform Immunohistochemistry PDGFβR - Rabbit Mouse -NA-

Get tips on using anti-Mucin 6, Clone: CLH5, Invitrogen™ to perform Immunohistochemistry Human - MUC-6

Get tips on using Androgen Receptor (D6F11) XP® Rabbit mAb #5153 to perform Immunohistochemistry Human - AR

Get tips on using Monoclonal Mouse Anti-Human Hepatocyte (Concentrate) Clone OCH1E5 to perform Immunohistochemistry Mouse - Hepatocyte

Get tips on using Donkey anti-rabbit IgG to perform Immunohistochemistry Anti-rabbit IgG - Donkey Rabbit Rhodamin red

Get tips on using Donkey anti-mouse IgG to perform Immunohistochemistry Anti-mouse IgG - Donkey Mouse Rhodamin red

Get tips on using Ki-67 Antigen, Clone MIB-1 to perform Immunohistochemistry Ki67 - Rabbit Mouse / Human -NA-

Get tips on using Purified Mouse Anti-Human MLH1 Clone G168-728 (RUO) to perform Immunohistochemistry Human - MLH1