Protein Expression Prokaryotic cells L. lactis

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Get tips on using Rat C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Rat - C-Reactive Protein/CRP

Products R&D Systems Rat C-Reactive Protein/CRP DuoSet ELISA

Get tips on using Human C-Reactive Protein/CRP DuoSet ELISA to perform ELISA Human - C-Reactive Protein/CRP

Products R&D Systems Human C-Reactive Protein/CRP DuoSet ELISA

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Scheffersomyces (Pichia) stipitis

Products Thermo Fisher Scientific Y-PER™ Yeast Protein Extraction Reagent

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Mouse prostate tissue

Products Thermo Fisher Scientific T-PER™ Tissue Protein Extraction Reagent

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Synechocystis sp (6803)_Cyanobacteria

Products Thermo Fisher Scientific B-PER™ Bacterial Protein Extraction Reagent

Get tips on using T-PER™ Tissue Protein Extraction Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

Products Thermo Fisher Scientific T-PER™ Tissue Protein Extraction Reagent

Get tips on using pPICZα A, B, & C Pichia Vectors to perform Protein expression and purification Yeast - Pichia pastoris EDIII-D1

Products Thermo Fisher Scientific pPICZα A, B, & C Pichia Vectors

Get tips on using HiTrap Q FF anion exchange chromatography column to perform Protein expression and purification Bacteria - Bacillus subtilis GCSF

Products GE Healthcare Life Sciences HiTrap Q FF anion exchange chromatography column

Proteins Protein tag His-tag removal

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat mesenchymal stem cells (rMSC)

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