dna-methylation-profiling-gene-specific-profiling-hela-p16

Get tips on using QIAamp DNA FFPE Tissue Kit to perform DNA isolation / purification Tissue - liver

Get tips on using Quick-DNA™ FFPE Kit to perform DNA isolation / purification Tissue - kidney

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - colon

Get tips on using AllPrep DNA/RNA Mini Kit to perform DNA isolation / purification Tissue - spleen

Get tips on using TaKaRa MiniBEST Universal Genomic DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines SH-SY5Y

Get tips on using TaKaRa MiniBEST Universal Genomic DNA Extraction Kit to perform DNA isolation / purification Cells - Immortalized cell lines HEK 293T

miRNA is the inherent gene silencing machinery which can have more than one mRNA target, whereas siRNA can be designed to target a particular mRNA target. By design, both siRNA and miRNA are 20-25 nucleotides in length. The target sequence for siRNAs is usually located within the open reading frame, between 50 and 100 nucleotides downstream of the start codon. There are two ways in which cells can be transfected with desired RNAi: 1. Direct transfection (with calcium phosphate co-precipitation or cationic lipid mediated transfection using lipofectamine or oligofectamine), and 2. Making RNAi lentiviral constructs (followed by transformation and transduction). Lentiviral constructs are time consuming, but provide a more permanent expression of RNAi in the cells, and consistent gene silencing. Direct transfection of oligonucleotides provides temporary genetic suppression. Traditional methods like calcium phosphate co-precipitation have challenges like low efficiency, poor reproducibility and cell toxicity. Whereas, cationic lipid-based transfection reagents are able to overcome these challenges, along with applicability to a large variety of eukaryotic cell lines. When using oligos, the ideal concentration lies between 10-50nM for effective transfection.

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using AllPrep DNA/RNA FFPE Kit to perform DNA isolation / purification Tissue - FFPE samples

Get tips on using EZ1 DNA Tissue Kit (48) to perform DNA isolation / purification Tissue - FFPE samples