siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat marrow stromal cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine marrow stromal cells

Get tips on using Silencer®_Faslg siRNA (r) to perform siRNA / miRNA gene silencing Rat - F98 Faslg

Get tips on using Rn_LOC312647_1_ ATG7 FlexiTube siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( ATG7

Get tips on using Silencer® Select_Alkbh1 siRNA (r) to perform siRNA / miRNA gene silencing Rat - B35 Alkbh1

Get tips on using Silencer® siRNA(m) Nostrin to perform siRNA / miRNA gene silencing Mouse - MS1 Nostrin

Get tips on using Oct-3/4 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - P19 Oct4

Get tips on using Silencer®_Skiv2l2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - P19 Skiv2l2

Get tips on using Estrogen Receptor alpha siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC ERα