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Protein isolation Bacteria - Synechocystis sp (6803)_Cyanobacteria Experiment

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Proteins Protein isolation Bacteria Synechocystis sp (6803)_Cyanobacteria

FastDNA™ SPIN Kit for Feces Product

Get tips on using FastDNA™ SPIN Kit for Feces to perform DNA isolation / purification Bacteria - Gram positive Clostridium difficile

Products MP Bio FastDNA™ SPIN Kit for Feces

Gentra Puregene Mouse Tail Kit (4 g) Product

Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies

Products Qiagen Gentra Puregene Mouse Tail Kit (4 g)

TRIzol Reagent Product

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human airway epithelial cells