Immunohistochemistry Anti-rabbit IgG Donkey

Get tips on using 53BP1 Antibody (H-300) to perform TissueFAxs 53BP1 [H-300] - Rabbit Human -NA-

Get tips on using 53BP1 Antibody (H-300) to perform Immunofluorscence  53BP1 [H-300] - Rabbit Human -NA-

Get tips on using Anti-Human FMC7 FITC/CD23 PE/CD19 PerCP-Cy™5.5 to perform Flow cytometry Anti-bodies Human - CD23

Get tips on using Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) to perform Flow cytometry Anti-bodies Mouse - CD16/CD32

Get tips on using Purified Mouse Anti-IKKγ Clone 54/IKKγ/NEMO (RUO) to perform Western blotting IKKgamma

Get tips on using Recombinant Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) to perform Western blotting VDAC1

Get tips on using Recombinant Anti-VDAC1 / Porin antibody [EPR10852(B)] - Mitochondrial Loading Control (ab154856) to perform Western blotting VDAC1

Get tips on using anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum to perform Autophagy assay cell type - CaCo-2

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).

Flow cytometry is an immunophenotyping technique whereby sing cell suspensions are stained for either cell surface markers or intracellular proteins by fluorescently-labelled antibodies and analyzed with a flow cytometer, where fluorescently-labelled molecules are excited by the laser to emit light at varying wavelengths, which is then detected by the instrument. There are several key criteria which are required to be kept in mind while designing a flow experiment- 1. Antibody titration (optimal dilution of antibodies should be calculated in order to avoid over- or under- saturated signals for proper detection of surface and intracellular markers), 2. Precision (3 or more replicates of the sample should be used per experiment), 3. Specificity (proper isotype controls should be included in the experiment), 4. Day-to-day variability (experiments should be repeated 3 or more times to ensure consistency and avoid variability due to flow cytometer settings), 5. Antibody interaction (Fluorescence minus one or FMO should be used, which is the comparison of signals from panel minus one antibody vs. the full panel), and 6. Antibody stability (fluorescently-labelled antibodies should be stored at 4C).