Protein Expression Prokaryotic cells A. tumefaciens

Get tips on using Chameleon® Vue Pre-stained Protein Ladder to perform Protein Ladder Immunofluorescence

Get tips on using BLUEstain™ Protein ladder, 11-245 kDa to perform Protein Ladder Prestained

Get tips on using Spectra™ Multicolor High Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Spectra™ Multicolor Low Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Blue Prestained Protein Marker Detection Pack #86810 to perform Protein Ladder Prestained

Get tips on using Chameleon® Kit Pre-stained Protein Ladders to perform Protein Ladder Prestained

Get tips on using Chameleon® Duo Pre-stained Protein Ladder to perform Protein Ladder Prestained

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) to perform Immunohistochemistry Anti-Glial Fibrillary Acidic Protein (GFAP) - Mouse Human -NA-