siRNA / miRNA gene silencing Mouse RGC-5

Get tips on using Slc1a2 to perform siRNA / miRNA gene silencing Rat - Glial cells GLT-1

Get tips on using SIHK1738 to perform siRNA / miRNA gene silencing Human - MDA-MB-231 PKN3

Get tips on using s4215 to perform siRNA / miRNA gene silencing Human - PANC-1 DNMT1/3b

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using MicroRNA isolation kit to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR

Get tips on using Silencer® Select_ MYD88 to perform siRNA / miRNA gene silencing Human - A375 MYD88

Get tips on using Silencer® Select_ TRIF to perform siRNA / miRNA gene silencing Human - A375 TRIF

Get tips on using esiRNA human PTPN3 (esiRNA1) to perform siRNA / miRNA gene silencing Human - A2780 PTPN3

Get tips on using ON-TARGETplus SMARTpool - Human to perform siRNA / miRNA gene silencing Human - U2OS DKC1

Get tips on using FlexiTube GeneSolution GS7052 for TGM2 to perform siRNA / miRNA gene silencing Human - Caki-2 TGM2