Western blot

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Western Blotting Protein Standards to perform Protein Ladder Immunofluorescence

Get tips on using Vimentin Monoclonal Antibody (V9), Biotin to perform Western blotting Vimentin

Get tips on using PCNA Antibody to perform Western blotting PCNA

Get tips on using eNOS antibody to perform Western blotting eNOS

Get tips on using SNAI1 antibody to perform Western blotting SNAI1

Get tips on using Oct4 antibody to perform Western blotting Oct4

Get tips on using ABCA1 Antibody to perform Western blotting ABCA1