DNA quantification Human

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The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Whole genome profiling MCF-7, MDA-MB-453 human breast cancer

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded

Products Promega QuantiFluor® dsDNA System

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification MCF10A breast epithelial cells

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Blood

Products Thermo Fisher Scientific Quant-iT™ PicoGreen® dsDNA Assay Kit

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human pulmonary artery smooth muscle cells (HPASMC)

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded

Products Thermo Fisher Scientific Qubit dsDNA HS Assay Kit

Get tips on using QuantiFluor® RNA System to perform RNA quantification Fuorimetric - human plasma

Products Promega QuantiFluor® RNA System

DNA DNA isolation / purification Cells Immortalized cell lines Human Neuroblastoma Cell Lines

Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Mouse - NIH 3T3

Products Thermo Fisher Scientific Quant-iT™ PicoGreen® dsDNA Assay Kit

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood

Products Thermo Fisher Scientific Qubit RNA HS Assay Kit

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