The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using QuantiFluor® dsDNA System to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification MCF10A breast epithelial cells
Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Blood
Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.
Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Colorectal aenocarenoma (SW48) - paraffin embeded
Get tips on using QuantiFluor® RNA System to perform RNA quantification Fuorimetric - human plasma
Get tips on using Quant-iT™ PicoGreen® dsDNA Assay Kit to perform DNA quantification Mouse - NIH 3T3
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood
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