Western blot Secondary Antibody Goat

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Goat Anti-Type III Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat Anti-Type I Collagen to perform Immunohistochemistry Collagen Type I - Goat Mouse -NA-

Get tips on using Goat Anti-Type III Collagen-BIOT to perform Immunohistochemistry Collagen Type III - Goat Mouse Biotin

Get tips on using Anti-LC3B antibody produced in rabbit to perform Western blot LC3B - Rabbit Human -NA-

Get tips on using BioMag Goat Anti-Rat IgG (500 ml) to perform Cell Isolation Mouse T cells

Get tips on using IRDye® 800CW Streptavidin to perform Western blot Biotin - -NA- -NA- Infrared dye 800CW

Get tips on using Anti-GATA4 Antibody to perform Western blotting GATA-4

Get tips on using Anti-GATA4 antibody (ab84593) to perform Western blotting GATA-4

Get tips on using Purified Mouse Anti-Nucleoporin p62 to perform Western blot p62/SQSTM1 - Mouse IgG2b Human -NA-