siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1

Get tips on using RealTime-Glo™ MT Cell Viability Assay to perform Live / Dead assay mammalian cells - INS-1 832/12

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using heat shock protein family A (Hsp70) member 5 to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) HSPA5 (GRP78)

Get tips on using Human SDC1 / Syndecan-1 ELISA Kit to perform ELISA Human - SDC1

Get tips on using Human MCP-1 ELISA Kit (ab179886) to perform ELISA Human - MCP1

Get tips on using Human Ubiquitin/Ubiquitin+1 Antibody to perform Western blotting Ubiquitin

Get tips on using NucleoSpin® miRNA to perform RNA isolation / purification Cells - primary human mesenchymal stem cells

Get tips on using Anti-Beclin 1 (Human) pAb to perform Autophagy assay cell type - UMR-106

Get tips on using Human TGF beta 1 ELISA Kit (ab100647) to perform ELISA Human - TGF-beta 1