CRISPR Mouse Deletion C2C12

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Get tips on using lentiGuide-Puro to perform CRISPR Human - Repression BIRC5

Get tips on using lentiGuide-Puro to perform CRISPR Human - Repression BIRC5

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression BCL6

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression PCSK9

Get tips on using pLX_311-Cas9 to perform CRISPR Human - Repression AKT2

Get tips on using dCas9 plasmid to perform CRISPR Human - Repression BRCA1

Get tips on using px330-mcherry to perform CRISPR Human - Repression HOTAIR

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Gene silencing through the use of small interfering RNA (siRNA) has become a primary tool for identifying disease-causing genes. There are several aspects for preparing and delivering effective siRNA to knockdown a target gene. The length of siRNA should be 21–23nt long with G/C content 30–50%. If a validated siRNA sequence for your target gene is not available, use siRNA generated against the entire target gene ORF. Always work with two or three different siRNA constructs to get reliable results. If you are not sure how much siRNA to use for a given experiment, start with a transfection concentration of 10-50 nM and use siRNA-specific transfection reagent to ensure efficient siRNA delivery in a wide range of cells.