DNA Damage Assay Human bronchial epithelial cells (hBE)

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - Nb2

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Nb2

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Podocytes

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - L929

Get tips on using Micro BCA™ Protein Assay Kit to perform Protein quantification Mammalian cells - 3T3

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - C2C12

Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - RAW264.7

Get tips on using Pierce™ BCA Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

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As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.