Immunohistochemistry Anti-mouse IgG

Get tips on using CD14 Monoclonal Antibody (Sa2-8), FITC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD14

Get tips on using FOXP3 Monoclonal Antibody (FJK-16s), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - FOXP3

Get tips on using FOXP3 Monoclonal Antibody (FJK-16s), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - FOXP3

Get tips on using CD3e Monoclonal Antibody (145-2C11), Biotin, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD3

Get tips on using CD11c Monoclonal Antibody (N418), PE-Cyanine5.5, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD11c

Get tips on using CD11b Monoclonal Antibody (M1/70.15), PE-Texas Red to perform Flow cytometry Anti-bodies Mouse - CD11b

Get tips on using ON-TARGETplus Mouse Jun siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Jun

Get tips on using ON-TARGETplus Mouse Gpam siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Gpat1

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.