Site Directed Mutagenesis (SDM) Human Deletion MCF-7

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Human VEGF PicoKine™ ELISA Kit to perform ELISA Human - VEGF

Get tips on using Human Sonic Hedgehog ELISA Kit (ab100639) to perform ELISA Human - ShhN

Get tips on using Human Prolactin PicoKine™ ELISA Kit to perform ELISA Human - PRL

Get tips on using Human Osteopontin (OPN) Quantikine ELISA Kit to perform ELISA Human - OPN

Get tips on using Human Myeloperoxidase ELISA Kit (MPO) (ab119605) to perform ELISA Human - MPO

Get tips on using Human MMP-9 Quantikine ELISA Kit to perform ELISA Human - MMP9

Get tips on using Human MMP9 ELISA Kit (lyophilized) (ab100610) to perform ELISA Human - MMP9

Get tips on using Human Leptin PicoKine™ ELISA Kit to perform ELISA Human - Leptin

Get tips on using Human LBP PicoKine™ ELISA Kit to perform ELISA Human - LBP