cDNA synthesis Bacteria

Get tips on using Sodium bisulfite to perform PCR Methylation specific PCR - Bacterial DNA

Get tips on using HaeIII Methyltransferase to perform PCR Methylation specific PCR - Bacterial DNA

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using CIRCLEGROW AGAR (POWDER) to perform Bacterial cell culture media Escherichia coli

Get tips on using CHROMagar™ Vibrio to perform Bacterial cell culture media Vibrio cholera

Get tips on using MUELLER-HINTON BROTH to perform Bacterial cell culture media Bordetella bronchiseptica

Get tips on using Mannitol Salt Agar to perform Bacterial cell culture media Staphylococcus saprophycitius

Get tips on using TRYPTIC SOY BROTH to perform Bacterial cell culture media Staphylococcus epidermidis

Get tips on using CHROMID® VRE to perform Bacterial cell culture media Enterococcus faecium

Get tips on using VRESelect Medium #63751 to perform Bacterial cell culture media Enterococcus faecium