protein-expression-and-purification-bacteria-escherichia-coli-p19

Get tips on using pKS2-ST::CBHII to perform Protein Expression Eukaryotic cells - W. anomalus CBHII

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HepG2

Get tips on using RIPA Lysis and Extraction Buffer to perform Protein isolation Mammalian cells - HEK293T

Get tips on using pGETS118 to perform Protein Expression Prokaryotic cells - B. subtilis cellulosomal complexes

Get tips on using pHYαCGT7 to perform Protein Expression Prokaryotic cells - B. subtilis α-CGTase

Get tips on using pHYαCGT1 to perform Protein Expression Prokaryotic cells - B. subtilis β-CGTase

Get tips on using pAF3 to perform Protein Expression Prokaryotic cells - B. subtilis PHY US417

Get tips on using pONE-30A to perform Protein Expression Eukaryotic cells - S. frugiperda MBP

Get tips on using AcP(−)p6.9hSEAP to perform Protein Expression Eukaryotic cells - S. frugiperda hSEAP