CRISPR Mouse Deletion B117P

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Deletion SKOV3 APRIN

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression HBV RT

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Activation α-synuclein

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression miR-21

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation ASCL1

Get tips on using lenti dCAS-VP64_Blast to perform CRISPR Human - Activation RhoGDIα

Get tips on using M-ST1n-VP64 to perform CRISPR Human - Activation RHOXF2