siRNA / miRNA gene silencing Mouse RGC-5

Get tips on using BfuI (BciVI) (5 U/µL) to perform Restriction Enzymes BciVI / BfuI

Get tips on using EndoFree Plasmid Mega Kit (5) to perform DNA isolation / purification Plasmid purification

Get tips on using pIEX-5-6His-mMBP-UMODpXR to perform Protein Expression Eukaryotic cells - HEK293 mMBP

Get tips on using GR Antibody (G-5): sc-393232 to perform Immunohistochemistry Human - GR/glucocorticoid receptor

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using T4 DNA Ligase (5 U/µL) to perform DNA ligation

Get tips on using Vimentin Antibody (E-5): sc-373717 to perform Western blotting Vimentin

Get tips on using Anti-SMAD1/5/9 antibody (ab66737) to perform Western blotting Smad1

Get tips on using p21 Antibody (F-5): sc-6246 to perform Western blotting p21

Get tips on using Lamin B1 Monoclonal Antibody (L-5) to perform Western blotting Lamin B