CRISPR Mouse Deletion 3T3-L1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Get tips on using lentiGuide-Puro to perform CRISPR Human - Repression BIRC5

Get tips on using lentiGuide-Puro to perform CRISPR Human - Repression BIRC5

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression BCL6

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression PCSK9

Get tips on using pLX_311-Cas9 to perform CRISPR Human - Repression AKT2

Get tips on using dCas9 plasmid to perform CRISPR Human - Repression BRCA1

Get tips on using px330-mcherry to perform CRISPR Human - Repression HOTAIR

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1