Immunofluorscence  53BP1 [H-300]

Get tips on using HinfI R6201 to perform Restriction Enzymes HinfI

Get tips on using FastDigest HindIII to perform Restriction Enzymes HindIII

Get tips on using HindIII R6041 to perform Restriction Enzymes HindIII

Get tips on using FastDigest Hin1II to perform Restriction Enzymes Hin1II / NlaIII

Get tips on using HaeIII R6171 to perform Restriction Enzymes HaeIII / BsuRI

Get tips on using FastDigest HpaII to perform Restriction Enzymes HpaII / MspI

Get tips on using HpaII R6311 to perform Restriction Enzymes HpaII / MspI

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.