siRNA / miRNA gene silencing Human HaCaT

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized HaCaT

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - HaCaT

Get tips on using siRNA Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - IEC Cationic lipid based

Get tips on using SPLInsert™ Hanging to perform Cell migration / Invasion cell type - HaCat

Get tips on using RIPA Lysis Buffer, 10X to perform Protein isolation Mammalian cells - HaCaT

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - HaCaT

Get tips on using LC3A/B Antibody #4108 to perform Autophagy assay cell type - HaCaT

Get tips on using AChE shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 AChE

Get tips on using MCM4 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - SiHa MCM4