CRISPR Mouse Deletion RMA cells

Get tips on using Cas9m4-VP64 to perform CRISPR Human - Activation NEUROD1

Get tips on using lentiGuide-Puro to perform CRISPR Human - Repression BIRC5

Get tips on using lentiGuide-Puro to perform CRISPR Human - Repression BIRC5

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression BCL6

Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression PCSK9

Get tips on using pLX_311-Cas9 to perform CRISPR Human - Repression AKT2

Get tips on using dCas9 plasmid to perform CRISPR Human - Repression BRCA1

Get tips on using px330-mcherry to perform CRISPR Human - Repression HOTAIR

Get tips on using phREX1-Luc to perform CRISPR Human - Activation REX1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.