Protein Expression Prokaryotic cells L. citreum

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Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion RNase L

Products Addgene pSpCas9(BB)-2A-GFP (PX458)

Get tips on using Mouse RANKL ELISA Kit (TNFSF11) (ab100749) to perform ELISA Mouse - RANK L

Products Abcam Mouse RANKL ELISA Kit (TNFSF11) (ab100749)

Get tips on using Anti-TATA binding protein TBP antibody [58C9] - Loading Control (ab61411) to perform Western blotting TBP

Products Abcam Anti-TATA binding protein TBP antibody [58C9] - Loading Control (ab61411)

Get tips on using Rat Retinol binding protein 4,RBP-4 ELISA Kit to perform ELISA Rat - RBP4

Products Cusabio Rat Retinol binding protein 4,RBP-4 ELISA Kit

Get tips on using Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897) to perform ELISA Human - RBP4

Products Abcam Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897)

Get tips on using Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit to perform ELISA Human - FABP2

Products Sigma-Aldrich Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit

Get tips on using Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit to perform ELISA Human - BRCA2

Products MyBioSource.com Human Breast Cancer Susceptibility Protein 2 (BRCA2) ELISA Kit

Get tips on using Prestained Dual Color Protein Molecular Weight Marker (1.7-40 kDa) Molecular Weight Marker to perform Protein Ladder Prestained

Products MyBioSource.com Prestained Dual Color Protein Molecular Weight Marker (1.7-40 kDa) Molecular Weight Marker

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Mouse L929 capsid protein

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