Protein expression and purification Insect cells Hi5

Get tips on using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents to perform Protein isolation Mammalian cells - HEK293T

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

Get tips on using PhosphoProtein Purification Kit (6) to perform Protein tag Purification of phosphorylated proteins

Get tips on using Maj-NPLP to perform Protein Expression Prokaryotic cells - E. coli M. japonicus neuroparsin-like peptide

Get tips on using pET-PvD1 to perform Protein Expression Prokaryotic cells - E. coli PvD1, from Phaseolus vulgaris L.

Get tips on using pTip-QC2-gi_21221697 to perform Protein Expression Prokaryotic cells - R. erythropolis putative gntR-family regulator

Get tips on using pSS1-EBA140RIII–V to perform Protein Expression Prokaryotic cells - L. lactis EBA140RIII–V P. falciparum

Get tips on using pPICZαA‐opt‐RABV‐G to perform Protein Expression Eukaryotic cells - P. pastoris opt‐RABV‐G