Stem cell Differentiation media

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Cellular assays Cell cytotoxicity / Proliferation assay cell type HCT116

Get tips on using LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells to perform Live / Dead assay mammalian cells - K562

Products Thermo Fisher Scientific LIVE/DEAD™ Cell-Mediated Cytotoxicity Kit, for animal cells

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Primary cells Rat mesenchymal stem cells (rMSC)

Products Thermo Fisher Scientific Lipofectamine® 2000 Transfection Reagent

Get tips on using Senescence Cells Histochemical Staining Kit to perform Reporter gene assay β-galactosidase substrates - human MSCs (mesenchymal stem cells)

Products Sigma-Aldrich Senescence Cells Histochemical Staining Kit

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cellular assays Cell cycle assay human MDA-MB-231

Get tips on using Beta-Glo® Assay System to perform Reporter gene assay β-galactosidase substrates - rat mesenchymal stem cells (MSCs)

Products Promega Beta-Glo® Assay System

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Products Sigma-Aldrich β-Gal Reporter Gene Assay, chemiluminescent

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Products Takara Bio Inc Luminescent β-galactosidase Detection Kit II

Cellular assays Cell line authentication MDA‐MB‐435 cell line

Cellular assays Cell line authentication Ovarian cancer cell line OVCAR3

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