rna-isolation-tissue

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Stomach

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Spleen

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Tissue - Rat Nose

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Lung

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Liver

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Kidney

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Intestine

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Heart

Get tips on using miRNeasy FFPE Kit to perform RNA isolation / purification Tissue - Rat Brain

Hello! I used Trizol to extract total RNA from in-vitro cultured bacteria (1 X 10^8 cells). After phase separation, I mixed ~0.4 ml of the upper phase which contains RNA with 0.5 mL cold isopropanol. However, the amount of RNA when measured in Nanodrop was very low. In addition, the ratio between 260 and 230 was around 0.1 to 0.5. Is there a chance that my sample was contaminated by the Trizol reagent? When I collected the aqueous phase I made sure to not touch the lower phase. What should I do?