siRNA / miRNA gene silencing Human Keratinocytes

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat epidermal keratinocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat epidermal keratinocytes

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Keratinocytes

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Keratinocytes

Get tips on using siRNA Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - IEC Cationic lipid based

Get tips on using AChE shRNA Plasmids (h) to perform shRNA gene silencing Human - TF‐1 AChE

Get tips on using MCM4 shRNA (h) Lentiviral Particles to perform shRNA gene silencing Human - SiHa MCM4

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - SHSY5Y Beclin 1

Get tips on using siRNA Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - IEC-6 Cationic lipid based