siRNA / miRNA gene silencing Human PANC-1

Get tips on using Human Syndecan-1 DuoSet ELISA to perform ELISA Human - SDC1

Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Human - PANC-1

Get tips on using MISSION® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles to perform shRNA gene silencing Human - Islets of langerhans Negative control (scrambled) lentiviral particles

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Human Ubiquitin/Ubiquitin+1 Antibody to perform Western blotting Ubiquitin

Get tips on using Click-iT™ EdU Pacific Blue™ Flow Cytometry Assay Kit to perform Cell cycle assay human - PANC-1

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / miRNA gene silencing Mouse - Glomerular mesangial cells HIPK2 Polymer / Lipid delivery

Get tips on using Human TGF beta 1 ELISA Kit (ab100647) to perform ELISA Human - TGF-beta 1

Get tips on using Human TGF-beta 1 Quantikine ELISA Kit to perform ELISA Human - TGF-beta 1