Immunohistochemistry CD3

Get tips on using C23 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Neuro 2a C23

Get tips on using Rat Fractalkine ELISA Kit (CX3CL1) (ab100760) to perform ELISA Rat - CX3CL1

Get tips on using Rat Fractalkine/CX3CL1 PicoKine™ ELISA Kit to perform ELISA Rat - CX3CL1

Get tips on using P-Cadherin (C13F9) Rabbit mAb #2189 to perform Western blotting P-Cadherin

Get tips on using Cy3 Mono-Reactive Dye Pack to perform Microarray RNA amplification & Labeling - Human brain tissue Cyanine 3

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - C13

Get tips on using Quick Amp Labeling Kit-one color to perform Microarray RNA amplification & Labeling - Mouse Myofibers Cy3- or/and Cy5