Protein Expression Prokaryotic cells C. glutamicum

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Get tips on using mRFP-green fluorescent protein (GFP)-LC3 adenoviral vector to perform Autophagy assay cell type - Macrophages

Products HanBio Technology mRFP-green fluorescent protein (GFP)-LC3 adenoviral vector

Get tips on using Blu10 Plus (BLUltra) Prestained Protein Ladder(6.5 to 270 kDa) to perform Protein Ladder Prestained

Products BIO-HELIX Blu10 Plus (BLUltra) Prestained Protein Ladder(6.5 to 270 kDa)

Get tips on using Blue Prestained Protein Marker, Broad Range (11-250 kDa) #59329 to perform Protein Ladder Prestained

Products Cell Signaling Technology Blue Prestained Protein Marker, Broad Range (11-250 kDa) #59329

Get tips on using Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028) to perform Protein Ladder Prestained

Products Abcam Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Get tips on using AllPrep Bacterial DNA/RNA/Protein Kit (50) to perform DNA isolation / purification Bacteria - Gram negative Massilia sp

Products Qiagen AllPrep Bacterial DNA/RNA/Protein Kit (50)

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human chondrocytes

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat cardiomyocytes

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human astrocytes

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human osteoblasts

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