Get tips on using Stat5b Antibody (C-8): sc-377069 to perform ChIP Anti-bodies Stat5b
Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.
Get tips on using CD52 Monoclonal Antibody (CF1D12), PE to perform Flow cytometry Anti-bodies Human - CD52
Get tips on using CD70 Monoclonal Antibody (BU69), FITC to perform Flow cytometry Anti-bodies Human - CD70
Get tips on using GATA-3 Antibody (HG3-31): sc-268 to perform ChIP Anti-bodies GATA3
Get tips on using CD126 antibody | B-R6 to perform Flow cytometry Anti-bodies Human - CD126/IL-6Ralpha
Get tips on using Epithelial Antigen, Ber-EP4, Unconjugated to perform Flow cytometry Anti-bodies Human - CD326/EpCAM
Get tips on using F4/80 antibody | Cl:A3-1 to perform Flow cytometry Anti-bodies Mouse - F4/80
Get tips on using CD23 Monoclonal Antibody (EBVCS2), eBioscience™ to perform Flow cytometry Anti-bodies Human - CD23
Get tips on using CD14 Monoclonal Antibody (61D3), eBioscience™ to perform Flow cytometry Anti-bodies Human - CD14
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