When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell
Get tips on using E.Z.N.A.® Total RNA Kit I to perform RNA isolation / purification Cells - primary bovine umbilical vein endothelial cells
Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Tissue - Human Gallbladder
Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Cells - immortalized C2C12
Get tips on using RoboGene® HDV RNA Quantification Kit 2.0 to perform RNA quantification qPCR
Get tips on using AllPrep DNA/RNA Mini Kit to perform AAA for reviews
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment