Protein Expression Prokaryotic cells A. cellulolyticus

Get tips on using Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027) to perform Protein Ladder Prestained

Get tips on using Prestained Protein Ladder – Extra broad molecular weight (6.5 – 270 kDa) (ab234592) to perform Protein Ladder Prestained

Get tips on using Prestained Protein Ladder – Extra broad molecular weight (5 - 245 kDa) (ab116029) to perform Protein Ladder Prestained

Get tips on using Human Retinol binding protein 4 ELISA Kit (RBP4) (ab108897) to perform ELISA Human - RBP4

Get tips on using Human FABP2 / Fatty Acid-Binding Protein, Intestinal ELISA Kit to perform ELISA Human - FABP2

Get tips on using Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa) #13070 to perform Protein Ladder Prestained

Get tips on using Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949 to perform Protein Ladder Prestained

Get tips on using Color-coded Prestained Protein Marker, Broad Range (10-250 kDa) #74124 to perform Protein Ladder Prestained

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.