Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Cells - immortalized PC-3
Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media PC-3
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - PC-3
Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - PC-3
Get tips on using SurePrint G3 Human Gene Expression 8x60K v2 Microarray Kit to perform Microarray Human - PCOS
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase
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