Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using Anti-trimethyl-Histone H3 (Lys4) Antibody, clone MC315, rabbit monoclonal to perform ChIP Anti-bodies H3K4me3
Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK
Get tips on using Anti-Ctip1/BCL-11A antibody [14B5] (ab19487) to perform ChIP Anti-bodies CtIP/BCL11A
Get tips on using Histone H3K9ac antibody to perform ChIP H3K9Ac - Sheep Rat -NA-
Get tips on using Histone H2A.X antibody (pAb) to perform ChIP Anti-bodies γH2AX
Get tips on using Histone H4K20me1 antibody (mAb) to perform ChIP Anti-bodies H4K20me1
Get tips on using Histone H3K27ac antibody (pAb) to perform ChIP Anti-bodies H3K27ac
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Rat - Heart
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment