Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.
Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Rat - Spinal cord
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Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - MCF-7
Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Rat - Brain microvessels
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Get tips on using Bcl-11B (D6F1) XP® Rabbit mAb #12120 to perform ChIP Anti-bodies CtIP/BCL11A
Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - MDA-MB-231
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