Protein expression and purification Yeast Saccharomyces cerevisiae

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Pierce™ Coomassie (Bradford) Protein Assay Kit Product

Get tips on using Pierce™ Coomassie (Bradford) Protein Assay Kit to perform Protein quantification Mammalian cells - RAW264.7

Products Thermo Fisher Scientific Pierce™ Coomassie (Bradford) Protein Assay Kit
M-PER™ Mammalian Protein Extraction Reagent Product

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - HUVEC

Products Thermo Fisher Scientific M-PER™ Mammalian Protein Extraction Reagent
Pierce™ Cell Surface Protein Isolation Kit Product

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - HUVEC

Products Thermo Fisher Scientific Pierce™ Cell Surface Protein Isolation Kit
Anti-Clara Cell Secretory Protein Antibody Product

Get tips on using Anti-Clara Cell Secretory Protein Antibody to perform Flow cytometry Anti-bodies Mouse - CCSP

Products Sigma-Aldrich Anti-Clara Cell Secretory Protein Antibody
RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus Experiment

The challenge in isolating RNA from S. aureus cells is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads is considered to be the better alternative.

RNA RNA isolation / purification Bacteria Gram positive Staphylococcus aureus
RNA isolation / purification Bacteria - Gram positive Streptococcus pneumoniae Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.

RNA RNA isolation / purification Bacteria Gram positive Streptococcus pneumoniae
RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Mycobacterium tuberculosis
RNA isolation / purification Bacteria - Gram positive Enterococcus faecalis Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Enterococcus faecalis
RNA isolation / purification Bacteria - Gram positive Clostridium difficile Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Clostridium difficile
RNA isolation / purification Bacteria - Gram positive Clostridium tetani Experiment

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.

RNA RNA isolation / purification Bacteria Gram positive Clostridium tetani

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