Site Directed Mutagenesis (SDM) Mouse Point mutation 3T3-L1

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using ViralSEQ™ Mouse Minute Virus (MMV) Detection System to perform Cell Culture Contamination Detection Kit Virus

Get tips on using PE Mouse Anti-Human CD61 Clone VI-PL2 to perform Flow cytometry Anti-bodies Human - CD61

Get tips on using PE Mouse Anti-Human CD26 Clone M-A261 to perform Flow cytometry Anti-bodies Human - CD26

Get tips on using PE-Cy™7 Mouse Anti-Human CD10 to perform Flow cytometry Anti-bodies Human - CD10

Get tips on using PE-Cy™7 Mouse Anti-Human CD56 to perform Flow cytometry Anti-bodies Human - CD56

Get tips on using PE-Cy™7 Mouse Anti-Human CD117 to perform Flow cytometry Anti-bodies Human - CD117

Get tips on using PE-Cy™7 Mouse Anti-Human CD28 to perform Flow cytometry Anti-bodies Human - CD28

Get tips on using PE Mouse Anti-Human CD30 Clone Ber-H83 to perform Flow cytometry Anti-bodies Human - CD30

Get tips on using PE-Cy™7 Mouse Anti-Human CD41a to perform Flow cytometry Anti-bodies Human - CD41