Western blot Secondary Antibody Goat Rabbit

Get tips on using Granzyme B Monoclonal Antibody (NGZB), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - Granzyme B

Get tips on using F4/80 Monoclonal Antibody (BM8), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - F4/80

Get tips on using CD326 (EpCAM) Monoclonal Antibody (G8.8), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD326/EpCAM

Get tips on using anti-p62 Protein, C-Terminal Specific Polyclonal Antibody to perform Autophagy assay cell type - MDA-MB-231

Get tips on using Human Thrombopoietin R/Tpo R APC-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R

Get tips on using Human Thrombopoietin R/Tpo R PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD110/Thrombopoietin R

Get tips on using Human IL-3R alpha /CD123 PE-conjugated Antibody to perform Flow cytometry Anti-bodies Human - CD123/IL3-R

Get tips on using CD140a (PDGFRA) Monoclonal Antibody (APA5), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD140/PDGFR-α

Get tips on using Anti-Collagen Type VII Antibody, clone 32,-VII to perform Immunohistochemistry Collagen VII [II-32] - Mouse Human -NA-

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.