siRNA / miRNA gene silencing Mouse Neuro 2a

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using NeuroCult™ Basal Medium (Mouse & Rat) to perform 3D Cell Culture Media Mouse embryonic neurospheres

Get tips on using pRNAT-H1.1/Neo to perform shRNA gene silencing Mouse - 4T1 Integrin α6

Get tips on using QuikChange II XL Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Mouse - Neuroblastoma 2a dibasic site, R18Q

Get tips on using connexin 43 ShRNA to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Connexin 43 lentiviral particles

Get tips on using INTERFERin® to perform siRNA / RNAi /miRNA transfection Mouse - B16 Polymer / lipid

Get tips on using IL-4Rα shRNA (m) Lentiviral Particles to perform shRNA gene silencing Mouse - R221a IL4Rα

Get tips on using AM5770: pSilencer™ 3.1-H1 neo to perform shRNA gene silencing Mouse - CT26 OPN

Get tips on using pSilencer 2.1-U6 Hygro to perform shRNA gene silencing Human - Neuroblastoma cells (SH-SY5Y) Beclin 1