ChIP acH4 Rabbit Rat

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HK-2 cells

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG

Get tips on using p21 Waf1/Cip1 (12D1) Rabbit mAb #2947 to perform Western blotting p21

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using Ki67 antigen rabbit polyclonal antibody to perform Cell cytotoxicity / Proliferation assay cell type - malignant peripheral nerve sheath tumor

Get tips on using Anti-LC3B antibody produced in rabbit to perform Autophagy assay cell type - Proximal tubular cells (rPT)

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Rabbit Anti-Human CHK2 (NT) Affinity Purified pAb to perform Immunohistochemistry chk2 - Rabbit IgG Human -NA-

Get tips on using [KO Validated] SOX2 Rabbit pAb (A0561) to perform Immunohistochemistry Human - SOX2