reporter-gene-assay-luciferase-bhk-21-baby-hamster-kidney-cells

Get tips on using Purified Hamster Anti-Mouse TCR β Chain to perform Flow cytometry Anti-bodies Mouse - TCRbeta

Get tips on using Purified NA/LE Hamster Anti-Mouse CD40 to perform Flow cytometry Anti-bodies Mouse - CD40

Get tips on using PerCP-Cy™5.5 Hamster Anti-Mouse CD69 to perform Flow cytometry Anti-bodies Mouse - CD69

Get tips on using PE-Cy™7 Hamster Anti-Mouse CD11c to perform Flow cytometry Anti-bodies Mouse - CD11c

Get tips on using 3D-Gene® Mouse miRNA Oligo chip (ver.21) to perform Microarray Gene expression arrays - Mouse liver tissue Cyanine-3-CTP

Get tips on using CelLytic™ M to perform Protein isolation Mammalian cells - BHK-21

Get tips on using LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation to perform Live / Dead assay mammalian cells - BHK-21

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized BHK-21

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.