CRISPR Mouse

Get tips on using pMXs-IRES-Bsd Retroviral Expression Vector to perform CRISPR Human - Repression DDX3X

Get tips on using pHR-SFFV-KRAB-dCas9-P2A-mCherry to perform CRISPR Human - Repression MYC

Get tips on using pHR-SFFV-KRAB-dCas9-P2A-mCherry to perform CRISPR Human - Repression GATA1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using pAC154-dual-dCas9VP160-sgExpression to perform CRISPR Human - Activation HIV-1 5′ LTR

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression HPV-18 E6

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Repression HPV-18 E7

Get tips on using pRSFDuet™-1 DNA - Novagen to perform CRISPR Hamster - Deletion CHO-K1 COSMC

Get tips on using pRSFDuet™-1 DNA - Novagen to perform CRISPR Hamster - Deletion CHO-K1 FUT8

Get tips on using pX330-U6-Chimeric_BB-CBh-hSpCas9 to perform CRISPR Rat - Deletion INS-1 832/13 Ep300