ChIP H3K36Me3 Bovine Mouse

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using ChromaFlash Chromatin Extraction Kit to perform ChIP Rat - Heart

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse to perform Western blotting ERK

Get tips on using PARP-1 C-terminal antibody (pAb) to perform ChIP Anti-bodies PARP

Get tips on using Recombinant Anti-PARP1 antibody [E102] (ab32138) to perform ChIP Anti-bodies PARP

Get tips on using Anti-RPA32/RPA2 antibody [9H8] (ab2175) to perform ChIP Anti-bodies RPA

Get tips on using Sall4 Antibody (EE-30): sc-101147 to perform ChIP Anti-bodies Sall4

Get tips on using Anti-Estrogen Receptor Antibody, clone AER304 to perform ChIP Anti-bodies ERα

Get tips on using Anti-dimethyl-Histone H3 (Lys27) Antibody to perform ChIP Anti-bodies H3K27me2