Protein Expression Prokaryotic cells B. licheniformis

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Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Human lung fibroblasts (HLF)

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat dermal fibroblasts (rDF)

Proteins Protein isolation Tissue Human tissue C-MFPE samples

Get tips on using Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa) #13070 to perform Protein Ladder Prestained

Products Cell Signaling Technology Color-coded Prestained Protein Marker, Low Range (1.7-42 kDa) #13070

Get tips on using Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949 to perform Protein Ladder Prestained

Products Cell Signaling Technology Color-coded Prestained Protein Marker, High Range (43-315 kDa) #12949

Get tips on using Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027) to perform Protein Ladder Prestained

Products Abcam Prestained Protein Ladder – Mid-range molecular weight (10 - 180 kDa) (ab116027)

Get tips on using Wilms' Tumor 1 (WT1) Protein, Clone 6F-H2 to perform Immunohistochemistry Wilms Tumor 1 (WT1) - Rabbit Mouse -NA-

Products Dianova Wilms' Tumor 1 (WT1) Protein, Clone 6F-H2

Get tips on using heat shock protein family A (Hsp70) member 5 to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) HSPA5 (GRP78)

Products Thermo Fisher Scientific heat shock protein family A (Hsp70) member 5

Proteins Protein tag Detection of proteins containing phosphorylated threonine residues

Proteins Protein tag Detection of proteins containing phosphorylated serine residues

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